Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors¶
Why this mattered¶
TBD
Abstract¶
(no abstract available)
Related¶
- cite → A rapid alkaline extraction procedure for screening recombinant plasmid DNA — The M13mp18 and pUC19 vector paper relies on rapid alkaline lysis as a practical screening method for identifying recombinant plasmid clones.
- cite → Studies on transformation of Escherichia coli with plasmids — The cloning-vector paper uses high-efficiency E. coli plasmid transformation methods as the experimental basis for propagating pUC and M13 recombinant vectors.
- cite → DNA sequencing with chain-terminating inhibitors — The M13 vector paper supports Sanger chain-termination sequencing by providing improved single-stranded phage templates and vector sequences.
- enables ← A rapid alkaline extraction procedure for screening recombinant plasmid DNA — Rapid alkaline plasmid extraction enabled efficient screening and preparation of recombinant vectors such as M13mp18 and pUC19.
- enables ← DNA sequencing with chain-terminating inhibitors — Sanger chain-termination sequencing enabled determination of the nucleotide sequences of the M13mp18 and pUC19 cloning vectors.