Positional cloning of the mouse obese gene and its human homologue¶

Why this mattered¶

TBD

(no abstract available)

cite → Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease — The obese-gene cloning work relies on Chirgwin-style guanidinium RNA isolation to obtain intact RNA for gene expression analysis.
cite → DNA sequencing with chain-terminating inhibitors — The obese-gene cloning study uses Sanger chain-termination sequencing to determine the mouse obese gene and human homolog sequences.
enables ← Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease — Chirgwin's RNA isolation method enables obese-gene cloning by preserving high-quality RNA needed to detect and characterize gene expression.
enables ← DNA sequencing with chain-terminating inhibitors — Sanger chain-termination sequencing enables obese-gene cloning by making nucleotide-level identification of the mouse gene and human homologue possible.